Corning RPMI 1640 Medium — 500 mL, Pack of 6

Corning RPMI 1640 Medium — the standard growth medium for lymphocytes, hybridomas, and suspension cell cultures. Formulated with L-glutamine and phenol red pH indicator. Each pack contains six 500mL bottles.

RPMI 1640 was originally developed at Roswell Park Memorial Institute for culturing human leukocytes. It is now one of the most widely used media in cell biology, supporting growth of a broad range of mammalian cell lines including T cells, B cells, NK cells, macrophages, and many tumor cell lines. Typically supplemented with 10% FBS and 1% Penicillin-Streptomycin.

Manufacturing: Produced under cGMP and ISO 9001:2015 certified conditions
Sterility: Sterile-filtered through 0.1µm membranes and tested for bacterial and fungal contamination
Endotoxin: Tested to ensure low endotoxin levels suitable for cell culture applications
pH: Adjusted to physiological range (7.2–7.4) and verified prior to release
Osmolality: Within specification for mammalian cell culture requirements
Lot Traceability: Full lot documentation from manufacture through delivery

Temperature: Store per label instructions — PBS and HBSS at 15–25°C (room temperature), RPMI 1640 at 2–8°C (refrigerated)
Light Sensitivity: Protect media containing phenol red from prolonged light exposure
Shelf Life: Refer to lot-specific expiration date on the bottle label
Opened Containers: Use promptly after opening. Aseptic technique required to prevent contamination
Supplements: Add serum, antibiotics, or growth factors as needed for your specific cell line protocol

Cell Washing: PBS is the standard rinse solution for removing serum and trypsin residues during passaging
Cell Culture: RPMI 1640 supports growth of lymphocytes, hybridomas, and suspension cell lines
Balanced Salt Solutions: HBSS maintains physiological pH and osmolality during short-term cell handling, rinsing, and transport
Reagent Preparation: PBS serves as a base diluent for antibodies, enzymes, and staining solutions
Tissue Processing: Used in histology, immunohistochemistry, and tissue dissociation protocols

ISO 9001:2015 Certified Manufacturing — Consistent production quality under quality management systems
cGMP Compliant — Manufactured following current Good Manufacturing Practice guidelines
Certificate of Analysis — Provided for every lot with complete analytical data including pH, osmolality, sterility, and endotoxin testing
Lot Traceability — Full chain of custody documentation available

Same-Day Shipping: Orders placed before 2 PM PST ship the same business day
Carrier: UPS with tracking for all shipments
Free Shipping: On orders over $500
Temperature: PBS and HBSS ship at ambient temperature. RPMI 1640 ships with cold packs when ambient temperatures exceed 25°C
No Hazmat: Cell culture media and buffers are non-hazardous — no special shipping restrictions